Volvox Medium

UTEX sells Volvox Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Volvox Medium

Freshwater Medium | 6 < pH < 8

Micronutrients NO3 Biotin Vitamin B12

Volvox Medium

Modification of the original recipe. Suitable for most axenic strains of Volvox and some of Eudorina and Pandorina. Not suitable for xenic cultures. For additional modifications of Volvox Medium, see HEPES-volvox, MES-volvox, P49, and Volvox-Dextrose.

Directions

For 1 L Total

To approximately 950 mL of dH₂O, add the following components in the order listed (except vitamins) while stirring continuously.
Adjust the pH to 8.0
Bring total volume to 1 L with dH₂O.
- For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
Cover and autoclave medium.
Allow to cool and add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.

#
1
Component	Ca(NO₃)₂•4H₂O (Sigma C 5676)
Amount	1 mL/L
Stock Solution Concentration	11.8 g/100 mL dH₂O
Final Concentration	0.5 mM
2
Component	MgSO₄•7H₂O (Sigma 230391)
Amount	1 mL/L
Stock Solution Concentration	4 g/100 mL dH₂O
Final Concentration	0.16 mM
3
Component	Na₂glycerophosphate•5H₂O (Sigma G 6501 )
Amount	1 mL/L
Stock Solution Concentration	5 g/100 mL dH₂O
Final Concentration	0.16 mM
4
Component	KCl (Fisher P 217)
Amount	1 mL/L
Stock Solution Concentration	5 g/100 mL dH₂O
Final Concentration	0.67 mM
5
Component	Glycylglycine (Sigma G 1002)
Amount	10 mL/L
Stock Solution Concentration	5 g/100 mL dH₂O
Final Concentration	3.8 mM
6
Component	P-IV Metal Solution
Amount	6 mL/L
7
Component	Vitamin B₁₂
Amount	1 mL/L
8
Component	Biotin Vitamin Solution
Amount	1 mL/L

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Ca(NO₃)₂•4H₂O (Sigma C 5676)	1 mL/L	11.8 g/100 mL dH₂O	0.5 mM
2	MgSO₄•7H₂O (Sigma 230391)	1 mL/L	4 g/100 mL dH₂O	0.16 mM
3	Na₂glycerophosphate•5H₂O (Sigma G 6501 )	1 mL/L	5 g/100 mL dH₂O	0.16 mM
4	KCl (Fisher P 217)	1 mL/L	5 g/100 mL dH₂O	0.67 mM
5	Glycylglycine (Sigma G 1002)	10 mL/L	5 g/100 mL dH₂O	3.8 mM
6	P-IV Metal Solution	6 mL/L
7	Vitamin B₁₂	1 mL/L
8	Biotin Vitamin Solution	1 mL/L

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Volvox Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.