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P-IV Metal Solution Component Recipe

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, add the nutrients in the order listed while stirring continuously.
    • Note: The Na2EDTA should be fully dissolved before adding other components.
  2. Bring total volume to 1 L with dH2O.
  3. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA•2H2O
(Sigma ED255)		 0.75 g/L 2 mM
2 FeCl3•6H2O
(Sigma F-1513)		 0.097 g/L 0.36 mM
3 MnCl2•4H2O
(Baker 2540)		 0.041 g/L 0.21 mM
4 ZnCl2
(Sigma Z-0152)		 0.005 g/L 0.037 mM
5 CoCl2•6H2O
(Sigma C-3169)		 0.002 g/L 0.0084 mM
6 Na2MoO4•2H2O
(J.T. Baker 3764)		 0.004 g/L 0.017 mM

Note: Final concentration listed is for the stock solution.