Erdschreiber's Medium

UTEX sells Erdschreiber's Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Erdschreiber's Medium

Saltwater Medium (30 ppt) | 6 < pH < 8

Micronutrients Natural Seawater NO3

View Media Recipe

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$ 55.00

Erdschreiber's Medium

Modified from the original Plymouth seawater recipe. General purpose marine medium for xenic cultures [for bacteria-free cultures see reprints in Rosowski & Parker (1971)].

Directions

For 3 L Total

To 3 L of pasteurized filtered seawater (30 ppt) aseptically add each of the sterile components in the order specified.
Vigorously swirl the contents of the flask to mix thoroughly.
Store at refrigerator temperature.

#
1
Component	Pasteurized Seawater
Amount	3 L
2
Component	P-IV Metal Solution
Amount	36 mL/3 L
3
Component	NaNO₃ (autoclave before adding) (Fisher BP360-500)
Amount	10 mL/3 L
Stock Solution Concentration	0.7 M
Final Concentration	2.3 mM
4
Component	Na₂HPO₄•7H₂O (autoclave before adding) (Sigma S-9390)
Amount	10 mL/3 L
Stock Solution Concentration	0.02 M
Final Concentration	0.067 mM
5
Component	Soilwater: GR+ Medium
Amount	150 mL/3 L
6
Component	Vitamin B₁₂
Amount	3 mL/3 L

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Pasteurized Seawater	3 L
2	P-IV Metal Solution	36 mL/3 L
3	NaNO₃ (autoclave before adding) (Fisher BP360-500)	10 mL/3 L	0.7 M	2.3 mM
4	Na₂HPO₄•7H₂O (autoclave before adding) (Sigma S-9390)	10 mL/3 L	0.02 M	0.067 mM
5	Soilwater: GR+ Medium	150 mL/3 L
6	Vitamin B₁₂	3 mL/3 L

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Erdschreiber's Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.