Spirulina Solution 1 Recipe

To approximately 450 mL of dH₂O, add the following components in the order listed while stirring continuously.
Bring total volume to 500 mL with dH₂O.
Autoclave medium.

#	Component	Amount	Final Concentration
1	NaHCO₃ (Fisher S 233)	13.61 g/500 mL dH₂O	162 mM
2	Na₂CO₃ (Baker 3604)	4.03 g/500 mL dH₂O	38 mM
3	K₂HPO₄ (Sigma P 3786)	0.5 g/500 mL dH₂O	2.9 mM